Cortisol, Two Specimens
Use
The Cortisol, Two Specimens test is typically ordered to assess adrenal gland function and to diagnose conditions such as Cushing's syndrome, Addison's disease, and adrenal insufficiency. The test measures cortisol levels at two different times to provide a more comprehensive evaluation of cortisol production patterns, which can be influenced by various physiological and pathological factors.
Special Instructions
Please refer to the directions for Sequential Sampling. This test may exhibit interference when the sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.
Limitations
The test may be affected by biotin interference, leading to inaccurate results if the patient has consumed biotin supplements within the last 72 hours. Additionally, the results may vary if the physiological state of the patient changes, such as during times of stress or illness, which can affect cortisol levels. Furthermore, improper sample handling and storage can also result in compromised specimen integrity and inaccurate measurement.
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 43215-3
- 21223-3
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.7 mL
Container
Gel-barrier tube (preferred) or red-top tube
Collection Instructions
If a red-top tube is used, transfer separated serum to a plastic transport tube.
Patient Preparation
Patients should stop biotin consumption at least 72 hours before sample collection.
Storage Instructions
Room temperature