Cystic Fibrosis (CF) Lower Respiratory Culture
Use
For the isolation and identification of microörganisms associated with pulmonary disease in patients with cystic fibrosis
Special Instructions
Specimens from cystic fibrosis patients will often grow potential pathogens. However, it's important to ensure specimen quality, with a significant emphasis on obtaining thick mucus in sufficient quantity and avoiding contamination with saliva.
Limitations
Anaerobic culture is not appropriate from expectorated sputum. Screening based on Gram stain evaluation is not recommended, as the presence of specific organisms is considered clinically significant for cystic fibrosis, regardless of Gram stain results. The test may not determine all species of bacteria present and is limited by the inability to culture anaerobic bacteria from sputum.
Methodology
Culture-based
Biomarkers
LOINC Codes
- 623-9
- 623-9
Result Turnaround Time
6-8 days
Related Documents
For more information, please review the documents below
Specimen
Sputum
Volume
5 to 10 mL
Minimum Volume
1 mL
Container
Sterile screw-cap container
Collection Instructions
Brush teeth and rinse mouth well before collection. Collect specimen through deep cough into container. Ensure collection of sufficient quantity of thick mucus.
Patient Preparation
Remove dentures, rinse mouth, gargle with water, and cough deeply to expectorate sputum.
Storage Instructions
Refrigerate at 2°C to 8°C.
Causes for Rejection
Unlabeled specimen, name discrepancy, inappropriate transport device, leaking specimen, prolonged delay, expired transport