Glucose 6-Phosphate Dehydrogenase (G6PD), Quantitative, Whole Blood and Red Blood Cell Count (RBC)
Also known as: G6PD, Quantitative, Blood and RBC
Use
Evaluate glucose 6-phosphate dehydrogenase (G6PD) deficiency.G6PD deficiency, an X-linked disorder, is the most common enzymatic disorder of red blood cells in humans, affecting more than 400 million people worldwide. The clinical expression of G6PD variants encompasses a spectrum of hemolytic syndromes. Affected patients are most often asymptomatic, but many patients have episodic anemia, while a few have chronic hemolysis.
Special Instructions
Care should be taken to avoid exposure to oxidant drugs and chemicals in patients with G6PD deficiency. Lists of safe and unsafe drugs are available, but further consultation and reevaluation may be necessary after hemolytic episodes.
Limitations
False normal results may occur after hemolysis where young erythrocytes and reticulocytes with normal G6PD levels are predominant. Therefore, results may not accurately reflect G6PD deficiency immediately following a hemolytic episode. Testing should be repeated after three months for patients with recent hemolytic crises, especially in certain ethnic groups such as Caucasian females and Blacks of both sexes.
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 49502-8
- 789-8
- 49502-8
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
8 mL (4 mL in each of two tubes)
Minimum Volume
RBC: two 500-μL lavender-top Microtainer™ tubes filled to at least 50% of tube capacity; G6PD: one lavender-top (EDTA) tube, green-top (heparin) tube or yellow-top (ACD) tube (0.1 mL)
Container
Two lavender-top (EDTA) tubes; one green-top (heparin) tube and one lavender-top (EDTA) tube; or one yellow-top (ACD) tube and one lavender-top (EDTA) tube
Storage Instructions
RBC: Stable room temperature for 1 day or refrigerated for 72 hours. G6PD: Stable room temperature for 72 hours or refrigerated for seven days.
Causes for Rejection
RBC: Hemolysis; tube not filled with minimum fill volume; specimen drawn in any anticoagulant other than EDTA; specimens diluted or contaminated with IV fluid; clotted specimen; improper labeling; transfer tubes with whole blood; lavender-top (EDTA) tubes received with plasma removed; samples more than 72 hours old. G6PD: Frozen specimen; clotted specimen.