Gynecologic Pap Test (Image-guided), Liquid-based Preparation and Chlamydia/Gonococcus, NAA With Reflex to Human Papillomavirus (HPV) (Aptima®) When ASC-U, ASC-H, LSIL, HSIL, AGUS
Use
Diagnose primary or metastatic neoplasm; detect Chlamydia trachomatis and Neisseria gonorrhoeae. This test aids in the diagnosis of sexually transmitted HPV infection. High-risk HPV test is used for types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68, without differentiation of the individual type.
Special Instructions
Include pertinent clinical information such as date of birth, medical history, and any previous abnormal Pap results on the cytology test request form. In accordance with CLIA criteria, Pap tests showing reactive or reparative cellular changes, atypical squamous or glandular cells of undetermined significance, or cells in the premalignant or malignant category will be referred for pathologist review, incurring additional charges.
Limitations
Adequate cell population from ectocervical, endocervical, or vaginal sites is essential for evaluation. Excessive use of lubricating jelly can interfere with cytological examination. Liquid-based cytology samples are limited in volume available for reprocessing or additional testing, such as HPV testing. A negative result does not exclude HPV infection, as low infection levels or sampling errors can cause false negatives. This test detects only the 14 most common high-risk HPV types and not the specific type present. Testing for Chlamydia and Gonococcus requires special procedures at the time of collection, with insufficient sample volumes limiting post-submission additions.
Methodology
PCR-based (Nucleic Acid Amplification)
Biomarkers
LOINC Codes
- 47527-7
- 50387-0
- 50388-8
Result Turnaround Time
2-6 days
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
Not provided
Minimum Volume
Not provided
Container
ThinPrep® vial
Collection Instructions
Broom-like collection: Insert the brush into cervical os, rotate five times, rinse in PreservCyt solution. Brush/spatula technique: Insert brush into endocervical canal, rotate, rinse in ThinPrep vial, secure cap.
Patient Preparation
Avoid douching 48-72 hours prior to exam. Do not collect during or right after menstrual period.
Storage Instructions
Store at room temperature. Process Pap within 21 days, HPV within 21 days from ThinPrep vial, Chlamydia/Gonococcus within seven days, Aptima swab within 60 days.
Causes for Rejection
Improper collection, labeling issues, insufficient quantity, expired vial, frozen specimens, older than allowable days for processing, or use of incorrect swab for collection.