Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Graphical)
Also known as: HIV-1 Plasma Viremia, HIV-1 RNA by Real-time PCR, Quantitative
Use
Detect and quantitate HIV-1 in plasma
Special Instructions
Submit the patient's Social Security number for serial monitoring purposes. To order this test without the serial monitor service, use test 550430.
Limitations
The assay is capable of measuring a range of 20−10,000,000 copies/mL. Due to potential cross-reactivity, clinicians should be cautious when conducting this test on patients who have undergone CAR-T cell therapy involving lentiviral vectors, as some may share a target region with the test, potentially resulting in false positives.
Methodology
PCR-based (qPCR)
Biomarkers
LOINC Codes
- 20447-9
- 20447-9
- 29541-0
- 80563-0
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
3.5 mL
Minimum Volume
1.1 mL
Container
Lavender-top (EDTA) tube or plasma preparation tube (PPT™)
Collection Instructions
Centrifuge sample within 24 hours of collection. Transfer plasma to a screw-cap polypropylene transport tube. Ship frozen (preferred). To avoid delays in turnaround time, submit separate frozen specimens for each requested test if multiple tests are ordered.
Storage Instructions
Freeze (preferred) or refrigerate. Stable at room temperature for 24 hours or refrigerated for six days. Stable frozen for six weeks.
Causes for Rejection
Incorrect anticoagulant; PPT™ frozen and/or not centrifuged
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 6 days |
| Frozen | 6 weeks |