Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Graphical) With Reflex to PhenoSense GT®
Also known as: HIV-1 Plasma Viremia, HIV-1 RNA by Real-time (RT) PCR, Quantitative, Reflex to PhenoSense GT®, Viral Load
Use
Detect and quantitate HIV-1 in plasma with reflex to PhenoSense GT® (at an additional charge) if viral load is >500 copies/mL.
Special Instructions
In the event that the viral load exceeds 500 copies/mL, the test reflexes to PhenoSense GT®, which involves additional testing that may incur extra charges. It is crucial for the specimen to be frozen for long-term storage. Separate frozen samples are required for the PhenoSense GT® portion of the test.
Limitations
The assay has a quantification range of 20−10,000,000 copies/mL. Specimens collected in green-top (heparin) tubes or received in certain types of transport tubes can result in rejection. Additionally, insufficient sample volume or improper storage and transport conditions can compromise the results. There may be delays in results due to the necessity of preparing separate frozen specimens when multiple tests are ordered from the same collection.
Methodology
PCR-based (qPCR)
Biomarkers
LOINC Codes
- 20447-9
- 20447-9
- 29541-0
- 80563-0
Result Turnaround Time
17-25 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
6 mL
Minimum Volume
4 mL
Container
Two or more screw-cap polypropylene frozen transport tubes
Collection Instructions
Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Remove plasma, transfer to screw-cap polypropylene transport tubes, and freeze. Ship frozen. Specimen transferred from PPT™ to screw-cap tubes prior to freezing.
Storage Instructions
Freeze. Frozen sample required for PhenoSense GT® portion. Stable at room temperature for one day, refrigerated for five days for the viral load assay. Long-term storage requires freezing.
Causes for Rejection
Hemolysis; use of green-top tube; improper tube type like 'pop-top' or 'snap-cap'; insufficient specimen quantity; frozen PPT™.