Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Graphical) With Reflex to PhenoSense GT®

Also known as: HIV-1 Plasma Viremia, HIV-1 RNA by Real-time (RT) PCR, Quantitative, Reflex to PhenoSense GT®, Viral Load

Clinical Use

Order Test

Use

Detect and quantitate HIV-1 in plasma with reflex to PhenoSense GT® (at an additional charge) if viral load is >500 copies/mL.

Special Instructions

Not provided.

Limitations

The assay has a quantification range of 20−10,000,000 copies/mL. Specimens collected in green-top (heparin) tubes or received in certain types of transport tubes can result in rejection. Additionally, insufficient sample volume or improper storage and transport conditions can compromise the results. There may be delays in results due to the necessity of preparing separate frozen specimens when multiple tests are ordered from the same collection.

Test Details

Methodology

PCR-based (qPCR)

Biomarkers

HIV-1

Microorganism

HIV-1 RNA

Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

LOINC Codes

Order Codes

20447-9 - HIV1 RNA # SerPl NAA+probe

Result Codes

20447-9 - HIV1 RNA # SerPl NAA+probe
29541-0 - HIV1 RNA SerPl NAA+probe-Log#
80563-0 - Report

Result Turnaround Time

17-25 days

Specimen

Plasma

Volume

6 mL

Minimum Volume

4 mL

Container

Two or more screw-cap polypropylene frozen transport tubes

Collection Instructions

Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Remove plasma, transfer to screw-cap polypropylene transport tubes, and freeze. Ship frozen. Specimen transferred from PPT™ to screw-cap tubes prior to freezing.

Storage Instructions

Freeze. Frozen sample required for PhenoSense GT® portion. Stable at room temperature for one day, refrigerated for five days for the viral load assay. Long-term storage requires freezing.