Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Nongraphical) With Reflex to HIV-1 GenoSure PRIme® or GenoSure Archive®
Also known as: HIV-1 Plasma Viremia, HiV-1 RNA Real-time PCR, Quantitative, Reflex to GenoSure Archive®, Reflex to GenoSure PRIme®, Viral Load
Use
Detect and measure HIV-1 in plasma with reflex to GenoSure PRIme® if viral load is greater than or equal to 500 copies/mL or reflex to GenoSure Archive® if viral load is less than 500 copies/mL. The GenoSure PRIme® and GenoSure Archive® assays detect mutations in the HIV viral genes that encode for HIV protease, reverse transcriptase and integrase. Mutations in these genes have been associated with resistance to antiretroviral drugs.
Special Instructions
If reflex tests are performed, additional charges or CPT codes may apply.
Limitations
This procedure should only be used for patients with a documented HIV-1 infection.
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 20447-9
- 20447-9
- 29541-0
- 51969-4
- 80689-3
- 62364-5
- 80695-0
- 8251-1
- 80693-5
- 80694-3
Result Turnaround Time
4-5 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
5 mL plasma
Minimum Volume
3.5 mL plasma
Container
Lavender-top (EDTA) tube, screw-capped polypropylene frozen transport tube
Collection Instructions
Centrifuge two tubes within six hours of collection. Remove plasma, transfer it to screw-cap polypropylene transport tubes, and freeze. Ship specimen frozen.
Storage Instructions
Freeze.
Causes for Rejection
Insufficient volume; heparinized plasma; specimen not frozen
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | 6 weeks |