Insulin, Two Specimens
Use
The Insulin, Two Specimens test is designed for assessing insulin levels at multiple points during a stimulation or suppression test. This test involves collecting a baseline serum specimen and additional specimens at various intervals after administering a treatment, such as a drug or glucose. It helps in evaluating insulin production dynamics, identifying potential insulin resistance, and assisting in the diagnosis of conditions such as insulinomas or hypoglycemia.
Special Instructions
Special instructions emphasize the importance of multiple specimen collection in the sequence specified, with correct time intervals. The test is unsuitable if no intervention (like medication or glucose) occurs between samples. Patients consuming high-dose biotin must avoid it 72 hours prior, as it may interfere with results. Label specimens accurately with patient name and time interval.
Limitations
This test may demonstrate interference in patients taking high doses of biotin (vitamin B7, B8, H, or coenzyme R). Precaution is needed to ensure continued biotin avoidance to avoid skewed results. The test should only be ordered when the treatment plan confirms an intervention is administered during the collection process. External factors including improper labeling, incorrect patient preparation, or handling of specimens may impact results.
Methodology
Immunoassay (CLIA)
Biomarkers
LOINC Codes
- 20448-7
- 46963-5
- 20448-7
- 46963-5
- 33816-0
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
2 mL
Minimum Volume
Not provided
Collection Instructions
Separate serum from cells within 45 minutes of venipuncture. Label each tube with patient name and time interval. Submit simultaneously from the same patient on one test request form.
Patient Preparation
Ask about biotin supplementation and ensure it is stopped at least 72 hours prior to sample collection.