Lactic Acid Dehydrogenase (LD)
Use
Elevated serum levels of LDH are observed in a variety of disease states. The highest levels are seen in patients with megaloblastic anemia, disseminated carcinoma, and shock. Moderate increases occur in muscular disorders, renal diseases, and cirrhosis. Mild increases in LDH activity have been reported in cases of myocardial or pulmonary infarction, leukemia, hemolytic anemia, and nonviral hepatitis.
Special Instructions
In infectious mononucleosis, LD is usually more elevated than AST, and there is usually an isomorphic pattern of LD isoenzymes. In viral hepatitis, AST and ALT (the transaminases) are much more increased than LD. Bovine or porcine heparin therapy can cause increases of AST, ALT, and LD, with elevated LD hepatic fractions.
Limitations
Hemolysis elevates LDH results, oxalate inhibits LDH, and ascorbic acid can decrease LDH values. Plasma specimens collected in EDTA, oxalate, or citrated tubes may be rejected. Gross hemolysis, gross bacterial contamination, or improper labeling are causes for rejection.
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 14804-9
- 14804-9
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.7 mL
Container
Red-top tube, gel-barrier tube
Collection Instructions
Separate serum or plasma from cells within 45 minutes of collection.
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Do not use oxalate, EDTA, or citrate plasma.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 7 days |
| Frozen | 14 days |