Myelin Basic Protein (MBP), Cerebrospinal Fluid
Also known as: MBP
Use
Assist in diagnosing multiple sclerosis; determine whether an MS patient is having an active demyelinating episode; determine whether active demyelination is occurring in patients being treated by intrathecal chemotherapy or radiation therapy for neoplastic diseases involving the central nervous system; diagnose acute brain tissue destruction in children, including newborns; assess the efficacy of potential treatments for MS
Special Instructions
Lumbar puncture should be performed within five days of the onset of neurological symptoms suggestive of multiple sclerosis. The test requisition form does not state FDA-approved use, and results are labeled for research purposes only by the assay's manufacturer. The results should not be used for diagnostic purposes without confirmation using another medically established diagnostic test.
Limitations
The presence of myelin basic protein (MBP) in cerebrospinal fluid may not always correlate with multiple sclerosis activity as the disease is often episodic. MBP levels can be low to undetectable between episodes, and are usually undetectable during remission. There's a possibility of normal MBP levels in MS patients during attack-free phases, and false elevations can occur in other disorders. For initial diagnosis of MS, alternative tests such as CSF oligoclonal bands and IgG index, which are positive in a majority of MS cases, are often preferred. Furthermore, MBP levels might help in assessing radiation-induced myelopathy and monitoring the impact of immunosuppressive therapy.
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 2638-5
- 2638-5
Result Turnaround Time
4-7 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
1 mL
Minimum Volume
0.8 mL
Container
Plastic transport tube
Collection Instructions
Centrifuge CSF at 1500xg for 20 minutes and place the supernatant in a separate tube. Ensure that residual fibrin and cellular matter have been removed prior to transport.
Patient Preparation
Lumbar puncture should be performed within five days of onset of neurologic symptoms suggestive of multiple sclerosis.
Storage Instructions
Room temperature
Causes for Rejection
Lipemic, hemolyzed, or icteric specimen; CSF should be free from contamination with blood. Hemolysis is associated with falsely-elevated levels of MBP.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |