PD-L1, IHC (Nivolumab)
Also known as: Opdivo®, Programmed Death Ligand 1
Use
The PD-L1, IHC test is used to evaluate programmed cell death-ligand 1 (PD-L1) expression in tumor tissues. PD-L1 is an immune inhibitory receptor ligand expressed by tumor cells and tumor-associated immune cells. This test is particularly significant for patients undergoing treatment with nivolumab, as PD-L1 expression on tumor cells can help predict response to therapy in cases of non-small-cell lung cancer. This immunohistochemical evaluation aids in the selection of suitable candidates for immune checkpoint inhibitors, potentially improving treatment outcomes by identifying tumors that may respond to nivolumab treatment.
Special Instructions
This test is specifically for tracking oncology IHC specimens to the laboratory using the test number 480588. Billing codes will be added post-testing based on laboratory processes completed. The tissue sample must be fixed in 10% neutral buffer formalin for no more than 72 hours and embedded in paraffin. Ensure the specimen is handled and processed according to standardized protocols for optimal results.
Limitations
The PD-L1, IHC (Nivolumab) test is limited by the quality and quantity of the specimen. Insufficient tissue or inappropriate fixation may affect test results and interpretations. Additionally, this assay is validated specifically for formalin-fixed paraffin-embedded (FFPE) tissue blocks or slides and might not be suitable for other types of specimens. It is important to note the test is applicable mainly to non-small-cell lung cancer and may not be validated for other tumor types.
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 85148-5
- 83055-4
- 83053-9
- 77202-0
- 72486-4
Result Turnaround Time
5-9 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
One formalin-fixed paraffin-embedded (FFPE) tissue block or five unstained positively charged slides sectioned from FFPE tissue block at 4 to 5 microns.
Minimum Volume
Three unstained tissue slides
Collection Instructions
Tissue should be fixed in 10% neutral buffered formalin for no more than 72 hours. Embed in paraffin.
Storage Instructions
Room temperature
Causes for Rejection
Insufficient tissue; tumor other than non-small-cell lung cancer