Pneumocystis Smear
Also known as: Pneumocystis carinii Stain, PCP (Pneumocystis carinii Pneumonia)
Use
Determine the presence of Pneumocystis carinii
Special Instructions
Noninduced expectorated sputum specimens are not recommended. Ensure specimens are stored and transported correctly to avoid rejection.
Limitations
The test cannot differentiate between colonization and active infection, so clinical correlation is essential. False negatives may occur due to the low organism load or suboptimal specimen collection. Swab specimens are not suitable for this test.
Methodology
Immunoassay (Direct or indirect fluorescent antibody (DFA))
Biomarkers
LOINC Codes
- 700-5
- 700-5
Result Turnaround Time
2-3 days
Related Documents
For more information, please review the documents below
Specimen
Sputum
Volume
5 to 10 mL induced sputum
Minimum Volume
Not provided
Container
Sterile leakproof specimen container
Collection Instructions
Noninduced expectorated sputum specimens are not recommended.
Storage Instructions
Refrigerate at 2°C to 8°C.
Causes for Rejection
Swab specimen; specimen received in grossly leaking transport container; specimen submitted in fixative or additive; inappropriate specimen transport conditions; specimen received after prolonged delay in transport (usually more than 72 hours); specimen stored or transported at room temperature; unlabeled specimen or name discrepancy between specimen and request label
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Prohibited |
| Refrigerated | 2 to 8°C |