T-Cell Receptor β-Chain Gene Rearrangements
Also known as: TCRB Gene Clonality Assay
Use
This assay can be used to detect clonal T-cell receptor β-chain gene rearrangements in blood, bone marrow, and tissue specimens. The presence of a monoclonal gene rearrangement usually, but not always, reflects the presence of a T-lymphocytic neoplasm, while polyclonal gene rearrangement patterns are found in benign reactive conditions. Thus, the results of these studies can assist in the diagnosis of lymphoproliferative disorders.
Special Instructions
Not provided.
Limitations
This PCR assay is able to detect a clonal DNA population at the sensitivity of 5% in a background of polyclonal DNA. This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 21751-3 - TCRB gene Rear Bld/T Ql
- 72486-4 - Lab director name Provider
- 49549-9 - Ref lab test method
Result Turnaround Time
5-7 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
3 to 5 mL
Minimum Volume
0.5 mL
Container
Lavender-top (EDTA) tube, yellow-top (ACD) tube, green-top (heparin) tube, tan-top (K2-EDTA) tube and pink-top (K2-EDTA) tube
Storage Instructions
Maintain blood at 2°C to 8°C
Causes for Rejection
Frozen whole blood/bone marrow; quantity not sufficient for analysis; tumor tissue; broken or stained slides
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