T-Cell Receptor Gene Rearrangements Profile, γ and β
Also known as: TCRB Gene Clonality Assay, TCRG Gene Clonality Assay
Use
This profile can be used to detect clonal T-cell receptor γ-chain (TCRG) and β-chain (TCRB) gene rearrangements in blood, bone marrow, and tissue specimens with a combined T-cell clonality detection rate of 94% to 99%. The presence of a monoclonal gene rearrangement usually, but not always, reflects the presence of a T-lymphocytic neoplasm, while polyclonal gene rearrangement patterns are found in benign reactive conditions. Thus, the results of these studies can assist in the diagnosis of lymphoproliferative disorders.
Special Instructions
Not provided.
Limitations
The PCR assay detects a clonal DNA population at a sensitivity of 5% in a background of polyclonal DNA. It may not detect all possible T-cell receptor gene rearrangements. The test is not cleared or approved by the FDA, and additional time may be required for confirmatory tests.
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 21753-9 - TCRG gene Rear Bld/T Ql
- 49549-9 - Ref lab test method
- 72486-4 - Lab director name Provider
- 21751-3 - TCRB gene Rear Bld/T Ql
- 21753-9 - TCRG gene Rear Bld/T Ql
- 72486-4 - Lab director name Provider
- 49549-9 - Ref lab test method
Result Turnaround Time
5-7 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
3 to 5 mL
Minimum Volume
0.5 mL
Container
Lavender-top (EDTA) tube, yellow-top (ACD) tube, green-top (heparin) tube, tan-top (K2-EDTA) tube and pink-top (K2-EDTA) tube
Collection Instructions
Small samples (e.g., punch biopsies, fine-needle aspirate) are accepted. Fresh tissue should be placed in RPMI medium or shipped frozen on dry ice.
Storage Instructions
Maintain at 2°C to 8°C.
Causes for Rejection
Frozen whole blood/bone marrow; quantity not sufficient for analysis; tumor block containing no tumor tissue; broken or stained slides
Other tests from different labs that may be relevant