Tryptase
Also known as: Mast Cell Tryptase
Use
This test measures total tryptase (alpha and beta tryptase). Tryptase is the most abundant protein component of human mast cell secretory granules.1,2 Serum levels generally reflect the extent of mast cell activation either by IgE- or non-IgE-mediated mechanisms. Basophils also produce small amounts of tryptase.
Special Instructions
Tryptase is often ordered as part of the diagnostic evaluation for mastocytosis or unexplained severe allergic reactions. It is particularly relevant for assessing mast cell burden in hematological abnormalities and neoplasms. Patients with elevated baseline tryptase levels may have an increased risk of severe anaphylactic reactions, particularly in response to insect stings or drugs.
Limitations
The measurement of tryptase cannot solely confirm mastocytosis or anaphylaxis. Tryptase levels should be interpreted in the context of clinical findings, as non-mast cell related conditions may also influence serum levels. Factors such as the timing of blood sample collection in relation to symptom onset are critical, as tryptase levels peak within one to two hours and gradually decline over the next three to six hours.
Methodology
Immunoassay (ImmunoCAP®)
Biomarkers
LOINC Codes
- 21582-2
- 21582-2
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.7 mL
Minimum Volume
0.5 mL
Container
Gel-barrier tube or lavender-top (EDTA) or green-top (heparin) tube
Collection Instructions
Separate serum or plasma from cells and transfer to a plastic transport tube.
Storage Instructions
Refrigerate
Causes for Rejection
Gross hemolysis; lipemic samples
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 14 days |
| Frozen | 14 days |