VistaSeq® Hereditary Cancer Panel Without BRCA
Use
This assay is intended for patients with a family history consistent with an inherited cancer syndrome.
Special Instructions
Not provided.
Limitations
Sequencing is limited to the coding region and flanking splice sites (+/-10bp). Variants in noncoding regions, deep intronic variants, and changes in repetitive elements such as microsatellite repeats may not be detected. Mosaic variants, inversions, or some genomic rearrangements like transpositions may go undetected. The method could be impacted by allele drop-out if a rare variant is present at a primer site. Copy number analyses may not detect all genomic rearrangements, such as translocations or some exon rearrangements. The assay cannot determine the exact breakpoints of detected deletions or duplications, and pseudogenes may interfere with variant detection in certain genes like PMS2.
Methodology
NGS (Targeted)
Biomarkers
LOINC Codes
- 73977-1 - Cancer multigene analysis Bld/T
- 31208-2 - Specimen source
- 51968-6 - Gene dis anl interp-Imp
- 69548-6 - Genetic var assess
- 47042-7 - Counseling note
- 77202-0 - Laboratory comment Report
- 49549-9 - Ref lab test method
- 75608-0 - Citation Ref Lab Test
- 72486-4 - Lab director name Provider
- 51969-4 - Gene analysis narr rpt Doc
Result Turnaround Time
23-28 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
10 mL
Minimum Volume
7 mL
Container
Lavender-top (EDTA) tube or yellow-top (ACD) tube
Collection Instructions
Blood is collected by routine phlebotomy.
Storage Instructions
Room temperature
Causes for Rejection
Frozen specimen; leaking tube; clotted specimen; quantity not sufficient for analysis; grossly hemolyzed specimen; incorrect anticoagulant.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 60 days |
| Refrigerated | 60 days |
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