Vitamin B12 and Folates
Use
The Vitamin B12 and Folates test is essential for diagnosing deficiencies in Vitamin B12 and folic acid, which are critical for maintaining healthy nerve cells and producing DNA and RNA. Deficiencies can result in megaloblastic anemia and other neurological disorders. The test is particularly significant in identifying the cause of anemia and assessing individuals with malabsorption disorders, vegan diets, or those on long-term antacid therapy, which may lead to deficiencies.
Special Instructions
This test may exhibit interference if the sample is collected from individuals consuming high doses of biotin supplements. Biotin is also known as Vitamin B7, B8, Vitamin H, or coenzyme R. It is recommended to advise patients to stop biotin intake at least 72 hours before sample collection to avoid test interference.
Limitations
While this test accurately measures Vitamin B12 and folate levels, it may not detect all conditions associated with deficiencies or excess of these vitamins. Factors such as liver disease, myeloproliferative disorders, and certain medications may also affect levels, leading to incorrect interpretations. Additionally, this test might not reflect recent changes in diet or supplementation since serum levels can fluctuate. Caution is advised when interpreting results from patients with recent changes in dietary intake or supplement regimen.
Methodology
Immunoassay (ECLIA)
Biomarkers
LOINC Codes
- 96805-7
- 2132-9
- 2284-8
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1.5 mL
Minimum Volume
0.6 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
If using a red-top tube, transfer separated serum immediately to a plastic transport tube.
Storage Instructions
Room temperature or refrigerated
Causes for Rejection
Plasma specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 7 days |
| Frozen | 14 days |