Vitamin K1
Also known as: Phylloquinone
Use
For the assessment of vitamin K deficiency. Vitamin K deficiency may be induced by obstructive liver disease, obstructive icterus, malabsorption due to celiac disease, pancreatitis, diarrhea, and antibiotic abuse; may be used to treat blood clotting disorders, bone metabolism disorders, and hemorrhagic disorders of newborns.
Special Instructions
Patients are required to fast for 12 hours overnight before specimen collection. Consumption of foods rich in vitamin K or supplementation may affect the test results and elevate plasma concentrations of Vitamin K1.
Limitations
The test requires a fasting period of 12 hours. While lipemia does not interfere with the analytical measurement, Vitamin K1 levels correlate with lipoprotein concentrations, thus lipemic samples should be rejected. These elevated levels can prevent accurate evaluation for Vitamin K1 insufficiency. The test is not cleared or approved by the FDA and is considered an in-house developed test requiring careful handling, with specimens protected from light.
Methodology
Mass Spectrometry
Biomarkers
LOINC Codes
- 9622-2
- 9622-2
Result Turnaround Time
4-6 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1.0 mL
Minimum Volume
0.5 mL
Container
EDTA (lavender-top) plasma, preferred. Also acceptable are lithium heparin (green-top) plasma, gel-separated (SST) serum, and standard (red-top) serum.
Collection Instructions
The blood should be collected by venipuncture into a Vacutainer® tube and mixed gently. Allow serum to clot for 10 minutes before centrifugation. Specimens must be protected from light and transferred to a labeled amber tube.
Patient Preparation
Patient should fast for 12 hours.
Storage Instructions
Specimens should be light-protected and shipped at room temperature or refrigerated (preferred).
Causes for Rejection
Receipt of mild or grossly lipemic specimen; receipt of grossly hemolyzed specimen; receipt of specimen not protected from light; receipt of specimen outside of stability; receipt of citrate plasma
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 14 days |
| Frozen | 28 days |