21-Hydroxylase Antibodies, Serum
Use
Investigating adrenal insufficiency and aiding in the detection of individuals at risk of developing autoimmune adrenal failure are key purposes of this test. Addison disease is the most frequent cause of primary adrenal insufficiency, and 21-hydroxylase autoantibodies are present in up to 90% of cases. This test is useful for evaluating the cause of established primary adrenal insufficiency and identifying those who may develop autoimmune adrenal failure in the future.
Special Instructions
Testing for autoantibodies against 21-hydroxylase is recommended following confirmation of adrenal insufficiency to help differentiate between causes of primary adrenal insufficiency. Ship specimen frozen on dry ice. Centrifuge and aliquot serum into a plastic vial to remove from cells or gel before shipping.
Limitations
Results should be interpreted in the context of clinical symptoms and adrenal functional confirmatory tests. Lipemic or grossly hemolyzed serum should not be used in this assay. In rare cases, some individuals may have human anti-mouse antibodies (HAMA) that interfere with immunoassays, so caution is advised in result interpretation if there is a correlation issue with the clinical presentation.
FDA ApprovedNew York Approved
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 85363-0
- 85363-0
Result Turnaround Time
3-9 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.2 mL
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial to remove from cells or gel prior to shipping.
Causes for Rejection
Gross hemolysis, gross lipemia, gross icterus
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | 14 days |