25-Hydroxyvitamin D2 and D3, Serum
Use
This test is useful for diagnosing vitamin D deficiency, differentiating the causes of rickets and osteomalacia, monitoring vitamin D replacement therapy, and diagnosing hypervitaminosis D. It is the preferred initial test for assessing vitamin D status and most accurately reflects the body's vitamin D stores.
Special Instructions
Long term use of anticonvulsant medications may result in vitamin D deficiency and subsequent bone disease. Specimen collection requires centrifuging and aliquoting the serum into a plastic vial within 2 hours of collection.
Limitations
The test has not been cleared or approved by the US Food and Drug Administration. It is developed according to CLIA requirements. The presence of conditions such as kidney disease may require different testing (e.g., 1,25-dihydroxyvitamin D levels). Alternative diagnoses should be considered if test results are inconsistent with clinical suspicion.
New York Approved
Methodology
Mass Spectrometry (LC-MS/MS)
Biomarkers
LOINC Codes
- 49590-3
- 49054-0
- 1989-3
- 62292-8
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.25 mL
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial within 2 hours of specimen collection.
Causes for Rejection
Only gross lipemia, hemolysis, and icterus are acceptable.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 28 days |
| Frozen | 30 days |