Acanthamoeba species, Molecular Detection, PCR, Ocular
Use
Aids in the diagnosis of amebic keratitis in conjunction with clinical findings. Amebic keratitis is a painful, subacute corneal infection that can cause extensive scarring and blindness if untreated. This test detects Acanthamoeba species DNA, providing a more rapid result compared to culture, with similar sensitivity and specificity.
Special Instructions
Not provided.
Limitations
While the assay is designed to detect symptomatic infection with Acanthamoeba species, the common presence of these organisms in the environment means they may contaminate contact lenses and cases. Results should be used only for patients with a clinical history consistent with amebic keratitis. Inadequate specimen collection or improper storage may invalidate test results.
New York Approved
Methodology
PCR-based (qPCR)
Biomarkers
Acanthamoeba species
Microorganism
LOINC Codes
- 41429-2 - Acanthamoeba DNA Spec Ql NAA+probe
- 31208-2 - Specimen source
- 41429-2 - Acanthamoeba DNA Spec Ql NAA+probe
Result Turnaround Time
2-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
5-10 mm(3)
Minimum Volume
Not provided
Container
Sterile container
Collection Instructions
Submit tissue in a sterile container with 1 mL of sterile saline, minimal essential media (MEM), or viral transport media.
Causes for Rejection
Specimens containing scalpel blades will be canceled.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |