Aldolase, Serum
Use
The Aldolase, Serum test is useful for the detection of muscle diseases. Aldolase is an enzyme involved in the glycolysis pathway, crucial for muscle energy production. It catalyzes the conversion of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, important for energy generation, especially in muscle tissue. Elevated levels of aldolase can indicate muscle diseases such as Duchenne muscular dystrophy, dermatomyositis, polymyositis, and limb-girdle muscular dystrophy. While creatine kinase levels are more specific, aldolase can also be elevated in patients with myositis who have normal CK values.
Special Instructions
Grossly hemolyzed, lipemic, or icteric specimens are subject to rejection. Ensure collection, processing, and transportation of specimens are conducted within recommended timeframes and conditions.
Limitations
This test has not been cleared or approved by the FDA and is performed as a modification of the manufacturer's instructions, analyzed in compliance with CLIA standards. Elevated aldolase levels are not exclusive to muscle disorders and must be corroborated with clinical findings and other diagnostic tests for accurate diagnosis of muscle conditions.
New York Approved
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 1761-6
- 1761-6
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL
Container
Plastic vial
Collection Instructions
Within 1 hour of collection, centrifuge and aliquot serum into a plastic vial. Send refrigerated.
Causes for Rejection
Gross hemolysis, gross lipemia, gross icterus
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 60 days |