Antineutrophil Cytoplasmic Antibodies Vasculitis Panel, Serum

Casandra

Casandra Test Code MC48674Version 1 (DRAFT)

Performing Lab

Lab Mayo Clinic LabLab Test ID VASCCPT Codes 83516, 86036

Antineutrophil Cytoplasmic Antibodies Vasculitis Panel, Serum

Clinical Use

Use

Evaluating patients with clinical features of anti-neutrophil cytoplasmic antibody-associated vasculitis, specifically granulomatosis with polyangiitis, microscopic polyangiitis, and eosinophilic granulomatosis with polyangiitis. Patients with suspected ANCA-associated vasculitis should be evaluated for the presence of PR3-ANCA, MPO-ANCA and ANCA by IIF. ANCA by IIF should then be used in cases where there is a high degree of suspicion for GPA or MPA, but the PR3-ANCA and MPO-ANCA testing is negative. ANCA-associated vasculitides are characterized by a pauci-immune inflammation within the walls of small blood vessels.

Special Instructions

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen: Kidney Transplant Test Request, Renal Diagnostics Test Request, or General Test Request. Preferred collection container is serum gel. Acceptable container is red top.

Limitations

A positive result for proteinase 3 or myeloperoxidase anti-neutrophil cytoplasmic antibodies is not diagnostic for any ANCA-associated vasculitis and must be interpreted in the clinical context of the patient. Negative results for PR3-ANCA, MPO-ANCA, and ANCA by IIF do not exclude the possibility of ANCA-associated vasculitis. Antibodies specific for antigens other than PR3 and MPO may lead to nuclear, perinuclear, or cytoplasmic staining on ethanol-fixed neutrophils.

Test Details

FDA Approved

New York Approved

Methodology

Immunoassay (Multiplex Protein Panel)

Biomarkers

3 components

Myeloperoxidase Antibodies, IgG • Multiplex Protein PanelProteinase 3 Antibodies, IgG • Multiplex Protein PanelCytoplasmic Neutrophilic Antibody Test • Multiplex Protein Panel