Ascorbic Acid (Vitamin C), Plasma
Use
Vitamin C, also known as L-ascorbic acid, is an essential dietary component for humans. It is necessary for various physiological functions, including the enzymatic amidation of neuropeptides, synthesis of adrenal cortical steroid hormones, conversion of tropocollagen to collagen, and metabolism of tyrosine and folate. It acts as a powerful antioxidant and plays a significant role in detoxifying liver enzymes, preserving vitamin E potential, and blocking harmful nitrosamines. A deficiency leads to scurvy, characterized by weakness, fatigue, and structural tissue weakening.
Special Instructions
Specimen should be shipped frozen on dry ice in an amber vial to protect from light. If not ordering electronically, complete and send the General Request form with the specimen.
Limitations
Testing of nonfasting specimens or the use of vitamin supplements can lead to elevated plasma vitamin C levels. The reference values were established based on fasting samples. After vitamin C intake, plasma values peak within 3 to 6 hours. Consuming aspirin, contraceptives, or certain medications can increase vitamin C needs, potentially leading to higher levels.
New York Approved
Methodology
Mass Spectrometry (LC-MS/MS)
Biomarkers
LOINC Codes
- 1903-4
- 1903-4
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1 mL
Minimum Volume
0.5 mL
Container
Amber vial
Collection Instructions
Immediately place specimen on wet ice and process within 4 hours of collection. Centrifuge at 4 degrees C, aliquot plasma into amber vial to protect from light. Freeze plasma immediately at or below -60 degrees C, protected from light.
Patient Preparation
Fasting: 12 hours, required; infants should have specimen collected before next feeding.
Storage Instructions
Ship specimen frozen on dry ice in amber vial to protect from light.
Causes for Rejection
Gross hemolysis
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | 14 days |