Aspartate Aminotransferase (AST) (GOT), Serum
Use
Diagnosing and monitoring liver disease, particularly diseases resulting in a destruction of hepatocytes. Aspartate aminotransferase (AST) is found in high concentrations in liver, heart, skeletal muscle, and kidney. AST is present in both cytoplasm and mitochondria of cells. In cases involving mild tissue injury, the predominant form of AST is that from the cytoplasm. Severe tissue damage results in more of the mitochondrial enzyme being released. High levels of AST can be found in cases such as myocardial infarction, acute liver cell damage, viral hepatitis, and carbon tetrachloride poisoning.
Special Instructions
Not provided.
Limitations
Pyridoxal phosphate is a cofactor in the reaction and is necessary for enzyme activity. Slight to moderate elevation of AST is seen in muscular dystrophy, dermatomyositis, acute pancreatitis, and crushed muscle injuries. Elevated AST values may also be seen in disorders affecting the heart, skeletal muscle, and kidney.
FDA ApprovedNew York Approved
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
Aspartate Aminotransferase
Analyte
LOINC Codes
- 30239-8 - AST SerPl w P-5'-P-cCnc
- 30239-8 - AST SerPl w P-5'-P-cCnc
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.25 mL
Container
Plastic vial
Collection Instructions
Serum gel tubes should be centrifuged within 2 hours of collection. Red-top tubes should be centrifuged, and the serum aliquoted into a plastic vial within 2 hours of collection.
Causes for Rejection
Gross hemolysis
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 7 days |
| Frozen | 30 days |