B-Cell Acute Lymphoblastic Leukemia/Lymphoma (ALL), Children's Oncology Group Enrollment Testing, FISH, Varies
Use
This test is utilized for detecting recurrent common chromosome abnormalities associated with B-cell acute lymphoblastic leukemia/lymphoma (B-ALL) and BCR::ABL1-like acute lymphoblastic leukemia/lymphoma in patients being considered for enrollment in Children's Oncology Group (COG) clinical trials and research protocols. It serves as an adjunct to conventional chromosome studies in pediatric patients with B-ALL and Ph-like ALL being considered for enrollment in COG protocols. Additionally, this test is useful for evaluating specimens in which chromosome studies are unsuccessful. However, it should not be used to screen for residual B-ALL/LBL.
Special Instructions
Not provided.
Limitations
This test is not approved by the US Food and Drug Administration and is best used as an adjunct to clinical and pathologic information. Fluorescence in situ hybridization (FISH) is not a substitute for conventional chromosome studies, which can detect chromosome abnormalities associated with other hematologic disorders that would be missed in a targeted B-cell acute lymphoblastic leukemia/lymphoma FISH panel test. Bone marrow is the preferred specimen type for this FISH test. If bone marrow is not available, a blood specimen may be used if there are circulating malignant cells in the blood as verified by a hematopathologist. If no FISH signals are observed post-hybridization, the case will be released as lack of FISH results.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
LOINC Codes
- 102100-5 - B-cell ALL Bld/T FISH
- 50397-9 - Mol dx interp Bld/T Ql
- 69965-2 - Clinical cytogeneticist review
- 93356-4 - Cells w cytogenetic abnl Bld/T
- 62356-1 - Chrom analy result (ISCN)
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Bone Marrow
Volume
2 to 3 mL
Minimum Volume
1 mL
Container
Yellow top (ACD)
Collection Instructions
It is preferable to send the first aspirate from the bone marrow collection. Invert several times to mix bone marrow. Send bone marrow specimen in original tube. Do not aliquot.
Causes for Rejection
Fresh tissue
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |