B-Cell Lymphoblastic Leukemia/Lymphoma, FISH, Tissue
Use
This test is useful for detecting recurrent chromosome abnormalities associated with B-cell acute lymphoblastic leukemia/lymphoma (B-ALL/LBL) and Philadelphia chromosome-like acute lymphoblastic leukemia (Ph-like ALL). It is particularly utilized at diagnosis to monitor response to therapy by tracking known chromosome abnormalities in patients with B-ALL/LBL. The test employs fluorescence in situ hybridization (FISH) on paraffin-embedded specimens to identify these chromosomal changes.
Special Instructions
Not provided.
Limitations
This test is not FDA-cleared or approved and serves as an adjunct to clinical and pathological information. FISH assays may not succeed with non-formalin fixed or decalcified specimens; success rate for decalcified specimens is about 50%. The assay does not detect all possible chromosome abnormalities, so a negative result does not rule out the presence of neoplastic disorders.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
LOINC Codes
- 102100-5 - B-cell ALL Bld/T FISH
- 50397-9 - Mol dx interp Bld/T Ql
- 69965-2 - Clinical cytogeneticist review
- 93356-4 - Cells w cytogenetic abnl Bld/T
- 62356-1 - Chrom analy result (ISCN)
- 42349-1
- 31208-2 - Specimen source
- 80398-1 - Unique ID Current sample
- 85069-3 - Lab test method
- 48767-8 - Annotation comment Imp
- 62364-5 - Test performance info Spec
- 18771-6 - Provider signing name
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Submit formalin-fixed, paraffin-embedded tumor tissue block. Blocks with alternative fixation will be attempted but are less favorable. Decalcified specimens have a 50% success rate. Fresh tissue will be rejected.
Causes for Rejection
Fresh tissue is not acceptable.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |