B-Cell Lymphoblastic Leukemia/Lymphoma, FISH, Tissue
Use
This test is useful for detecting recurrent chromosome abnormalities associated with B-cell acute lymphoblastic leukemia/lymphoma (B-ALL/LBL) and Philadelphia chromosome-like acute lymphoblastic leukemia (Ph-like ALL). It is particularly utilized at diagnosis to monitor response to therapy by tracking known chromosome abnormalities in patients with B-ALL/LBL. The test employs fluorescence in situ hybridization (FISH) on paraffin-embedded specimens to identify these chromosomal changes.
Special Instructions
The test does not include a pathology consultation. If a consultation is needed, a separate order is required. It is not intended for testing blood and bone marrow specimens, and fresh tissue is rejected; only paraffin-embedded tissue is acceptable. Detailed instructions regarding required pathology reports and listed probe requests are necessary for processing the test.
Limitations
This test is not FDA-cleared or approved and serves as an adjunct to clinical and pathological information. FISH assays may not succeed with non-formalin fixed or decalcified specimens; success rate for decalcified specimens is about 50%. The assay does not detect all possible chromosome abnormalities, so a negative result does not rule out the presence of neoplastic disorders.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
LOINC Codes
- 102100-5
- 50397-9
- 69965-2
- 93356-4
- 62356-1
- 42349-1
- 31208-2
- 80398-1
- 85069-3
- 48767-8
- 62364-5
- 18771-6
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Submit formalin-fixed, paraffin-embedded tumor tissue block. Blocks with alternative fixation will be attempted but are less favorable. Decalcified specimens have a 50% success rate. Fresh tissue will be rejected.
Causes for Rejection
Fresh tissue is not acceptable.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |