B-Cell Lymphoma, FISH, Tissue
Use
This test is useful for detecting recurrent common chromosome abnormalities associated with various B-cell lymphomas at diagnosis in paraffin-embedded tissue specimens. Genetic abnormalities have emerged as important prognostic markers and can aid in diagnosis. Fluorescence in situ hybridization (FISH) permits the detection of recurrent gene rearrangements associated with various chromosome translocations and inversions in B-cell lymphoma.
Special Instructions
A pathology report is required for testing. Acceptable reports include working drafts, preliminary pathology, or surgical pathology reports. The report must include patient name, block number on all blocks, slides, and paperwork, date of collection, tissue source, and reason for testing.
Limitations
This test is not approved by the FDA and should be used as an adjunct to clinical and pathologic information. Fixatives other than formalin may not ensure successful FISH assays. Paraffin-embedded tissues that have been decalcified have approximate 50% success rate. Testing might be canceled if there is insufficient tumor tissue.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
LOINC Codes
- 101651-8
- 50397-9
- 69965-2
- 93356-4
- 62356-1
- 42349-1
- 31208-2
- 85298-8
- 80398-1
- 85069-3
- 48767-8
- 62364-5
- 18771-6
Result Turnaround Time
4-10 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Submit a formalin-fixed, paraffin-embedded tumor tissue block. Blocks with alternative fixation methods like Prefer or Bouin’s may be attempted but are less successful. Decalcified paraffin-embedded specimens have an approximate 50% success rate.
Causes for Rejection
Fresh tissue specimens will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient preferred |