Babesia species, Molecular Detection, PCR, Blood
Use
This test is crucial for initial screening or confirmatory testing for suspected babesiosis during the acute febrile stage of infection, particularly in patients from endemic areas. It is especially useful when Giemsa-stained peripheral blood smears do not reveal any organisms, or when the morphology of organisms is inconclusive. The test identifies the DNA of Babesia species, providing a more sensitive alternative to microscopic examination of blood smears.
Special Instructions
Not provided.
Limitations
The test is designed to detect symptomatic infections with Babesia microti, Babesia duncani, and Babesia divergens/MO-1. However, it may also detect asymptomatic parasitemia in endemic areas, thus it should be used in patients with symptoms and a clinical history consistent with babesiosis. Inhibitory substances may cause false-negative results, and the test does not cross-react with malaria parasites. It's essential to correlate positive results with blood smear microscopy, serological results, and clinical findings.
New York Approved
Methodology
PCR-based (Real-Time PCR)
Biomarkers
LOINC Codes
- 88461-9 - Babesia sp 18S rRNA Pnl Bld NAA+non-pr
- 88452-8 - B microti 18S rRNA Bld Ql NAA+n-prb
- 88451-0 - B duncani 18S rRNA Bld Ql NAA+n-prb
- 88450-2 - B div+MO-1 18S rRNA Bld Ql NAA+n-prb
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
1 mL
Minimum Volume
0.5 mL
Container
Lavender top (EDTA)
Collection Instructions
Send whole blood specimen in the original tube (preferred).
Causes for Rejection
Gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |