BCR/ABL1, p190, mRNA Detection, Reverse Transcription-PCR (RT-PCR), Quantitative, Monitoring Assay, Varies
Use
Monitoring response to therapy in patients with known e1/a2 BCR/ABL1 (p190) fusion forms is crucial for evaluating treatment efficacy, especially in patients with acute lymphoblastic leukemia (ALL) and a subset of individuals with acute myeloid leukemia (AML). Detecting the BCR/ABL1 fusion and its transcript levels helps in tracking the disease progression and response to therapy. This monitoring is essential for adjusting treatment plans and predicting outcomes in patients harboring the p190 fusion protein, which is predominant in a small subset of chronic myeloid leukemia (CML) cases and significant in childhood ALL cases.
Special Instructions
Refrigerate specimens must arrive within 5 days (120 hours) of collection, and ambient specimens must arrive within 3 days (72 hours) of collection. Collect and package specimen as close to shipping time as possible. Necessary information includes pertinent clinical history detailing if the patient has a diagnosis of chronic myeloid leukemia or other BCR/ABL1-positive neoplasm.
Limitations
This test detects only the e1/a2 BCR/ABL1 (p190) fusion form. Other fusion forms, such as those containing the BCR e13 and e14 exons coding for the p210 protein, are not detected by this assay. The precision of this assay at very low BCR/ABL1 levels is less reliable, with significant inter-run variation possible. Results from other PCR assays or using different laboratories cannot be directly compared due to variations in methodology. Also, results from this PCR-based assay are not comparable to those obtained using fluorescence in situ hybridization (FISH) technology, as FISH measures DNA alleles while this assay measures mRNA transcripts.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 21823-0
- 31208-2
- 69047-9
Result Turnaround Time
4-8 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
10 mL
Minimum Volume
8 mL
Container
Preferred: Lavender top (EDTA), Acceptable: Yellow top (ACD)
Collection Instructions
Invert several times to mix blood. Send whole blood specimen in original tube. Do not aliquot. Label as blood.
Causes for Rejection
Gross hemolysis, moderately to severely clotted
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 5 days |