Beta-Hydroxybutyrate, Serum
Use
The test is useful for monitoring therapy in diabetic ketoacidosis and for investigating differential diagnoses in patients presenting with hypoglycemia, acidosis, suspected alcohol ingestion, or unexplained increases in anion gap. In pediatric patients, the presence or absence of ketonemia or ketonuria is essential for diagnosing inborn errors of metabolism. Serum beta-hydroxybutyrate is also a key parameter during controlled 24-hour fasts.
Special Instructions
Normally, the ratio of beta-hydroxybutyrate to acetoacetate ranges between 3:1 and 7:1 in severe ketotic states. After fasting overnight, beta-hydroxybutyrate levels should not exceed 0.4 mmol/L. Fasting tests are not recommended for children under 2 years.
Limitations
The test should not be performed on patients under 2 years of age for 24-hour fasting assessments. Dipstick tests using nitroprusside reagent do not measure beta-hydroxybutyrate and may underestimate ketone body status.
FDA ApprovedNew York Approved
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 6873-4
- 6873-4
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.25 mL
Container
Serum gel (preferred), Red top acceptable, Plastic vial for submission
Collection Instructions
Serum gel tubes should be centrifuged within 2 hours of collection. Red-top tubes should be centrifuged, and the serum aliquoted into a plastic vial within 2 hours of collection.
Storage Instructions
Serum should be frozen (preferred) or refrigerated for up to 7 days.
Causes for Rejection
Gross hemolysis
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |