Bilirubin, Total, Serum
Use
Bilirubin is one of the most frequently used tests to assess liver function. It helps in evaluating a wide range of diseases affecting the production, uptake, storage, metabolism, or excretion of bilirubin. This includes conditions like hemolysis, ineffective erythropoiesis, obstruction, hepatitis, hereditary and neonatal jaundice, and Crigler-Najjar syndromes. The test is also useful for monitoring the efficacy of neonatal phototherapy.
Special Instructions
Not provided.
Limitations
Specimens should be protected from light and analyzed as soon as possible. Gross hemolysis can lead to false results due to hemoglobin interfering with the diazo reaction. Compounds competing for binding sites on serum albumin such as penicillin may lower serum bilirubin levels. Results from some multiple myeloma patients may show bias, impacting reliability.
New York Approved
Methodology
Automated Analyzer (Photometric)
Biomarkers
Bilirubin
Analyte
LOINC Codes
- 1975-2 - Bilirub SerPl-mCnc
- 1975-2 - Bilirub SerPl-mCnc
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.25 mL
Container
Amber vial
Collection Instructions
Serum gel tubes should be centrifuged within 2 hours of collection. Red-top tubes should be centrifuged, and the serum aliquoted into an amber vial within 2 hours of collection.
Storage Instructions
Store refrigerated for up to 7 days. If ambient, uphold for 24 hours, and for frozen storage, 30 days.
Causes for Rejection
Gross hemolysis
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 7 days |
| Frozen | 30 days |