Bone Histomorphometry, Gross Microscopic Exam
Use
Bone histomorphometry allows for a detailed analysis of bone architecture and turnover using full-thickness bone biopsy samples. It is particularly useful in diagnosing various metabolic bone diseases such as renal osteodystrophy, osteoporosis, osteomalacia, and aluminum toxicity, among others. The procedure includes the examination of stained bone sections to assess the bone formation rate and mineralization, providing insights into bone health and the efficacy of therapeutic interventions.
Special Instructions
For first-time users, consultation with a Mayo Clinic Laboratories pathologist or endocrinologist/nephrologist is recommended. The use of metal-free containers is mandatory to prevent contamination, and the specimen should be shipped in 70% ethanol to preserve the tetracycline label. Detailed collection instructions can be found in the Bone Histomorphometry Specimen Preparation PDF document.
Limitations
Accurate assessment of mineralization and bone formation rates is contingent upon the appropriate administration of tetracycline prior to biopsy. Improper specimen handling, such as using non-metal-free containers or incorrect fixation methods, can lead to specimen rejection or compromised results. Dairies should not be consumed simultaneously with tetracycline to avoid interference with its absorption.
Methodology
Other
Biomarkers
LOINC Codes
- 60570-9
- 59465-5
- 51628-6
- 19139-5
- 22633-2
- 80398-1
- 62364-5
Result Turnaround Time
26-35 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
5 mm bone
Container
Metal-free specimen vial
Collection Instructions
Fix specimen in 70% ethanol. Ensure metal-free containers are used to avoid contamination. The anterior iliac crest is the preferred biopsy site.
Patient Preparation
Ensure correct schedule of tetracycline administration before biopsy. Avoid dairy products at the time of tetracycline ingestion.
Storage Instructions
Ship in 70% ethanol to preserve the tetracycline label.
Causes for Rejection
Fixatives other than 70% alcohol, Decalcified bone
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |
| Refrigerated | Refrigerated acceptable |