BRAF and KIT Mutation Analysis, Next-Generation Sequencing, Tumor

Casandra

Casandra Test Code MC62816Version 1 (DRAFT)

Performing Lab

Lab Mayo Clinic LabLab Test ID BRFKTCPT Codes 81406, 81479, 88381

BRAF and KIT Mutation Analysis, Next-Generation Sequencing, Tumor

Clinical Use

Use

This test is useful for identifying specific mutations within the BRAF and KIT genes that predict response to targeted therapies in patients with melanoma. It helps in determining candidates who may benefit from therapies directed at pathways involving KIT and BRAF, which have shown success in improving progression-free and overall survival.

Special Instructions

A pathology report containing patient name, block number, tissue collection date, and source of tissue must accompany the specimen. This test will always include a slide review at an additional charge.

Limitations

This test cannot differentiate between somatic and germline alterations in the BRAF and KIT genes. It may not detect multi-exon deletions, duplications, larger scale genomic copy number variants, or epigenetic modifications. The analytical sensitivity requires at least 5% mutant allele frequency with a minimum of 500x coverage for detection.

Test Details

New York Approved