Busulfan, Intravenous Dose, Area Under the Curve, Plasma
Use
This test is used for guiding dosage adjustments of busulfan to achieve complete bone marrow ablation while minimizing dose-dependent toxicity before hematopoietic stem cell transplantation for chronic myelogenous leukemia. It measures the area under the curve (AUC) and clearance of busulfan to facilitate dose adjustment, ensuring adequacy of dosing and avoiding potential toxicity, such as veno-occlusive liver disease, seizures, and coma.
Special Instructions
Not provided.
Limitations
The AUC results are dependent on the correct collection and handling of the plasma specimens as described. Incorrect protocol execution may lead to inaccurate pharmacokinetic evaluations and potentially misguided dosing adjustments. Doe limit valid for specific dosing protocols (0.8 mg/kg every 6 hours) and may not apply to alternative dosing regimens or specimens collected outside the prescribed timeline. Gross hemolysis, lipemia, or icterus do not invalidate the test results.
New York Approved
Methodology
Mass Spectrometry (LC-MS/MS)
Biomarkers
Busulfan
Analyte
LOINC Codes
- 93478-6 - Busulfan AUC Pnl Plas
- 33882-2 - Collect Date
- 49049-0 - Collect Tme Spec
- 93436-4 - Busulfan immed p busulfan Plas-mCnc
- 93435-6 - Busulfan 1h p busulfan Plas-mCnc
- 93434-9 - Busulfan 2h p busulfan Plas-mCnc
- 93433-1 - Busulfan 4h p busulfan Plas-mCnc
- 93477-8 - Busulfan Gvn ?Tm Dose
- 88063-3 - Chemotherapy infusion start date
- 88060-9 - Chemo infusion start time Clock time
- 88062-5 - Chemotherapy infusion stop date
- 88061-7 - Chemo infusion stop time Clock time
- 29463-7 - Weight
- 30525-0 - Age
- 93476-0 - Busulfan Plas-AUC
- 93475-2 - Busulfan Cl from Plas
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1 mL
Minimum Volume
0.25 mL
Container
Plastic vials
Collection Instructions
Collect 4 plasma samples: one immediately post first intravenous busulfan infusion, and at 1, 2, and 4 hours post-infusion. Keep on wet ice or refrigerated, centrifuge within 2 hours, then separate plasma and store in labeled plastic vials. Freeze immediately at -20°C.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 72 hours |
| Frozen | 28 days |