C3a Level By RIA
Use
The C3a Level test is used to measure the amount of C3a, a component of the complement system, in the plasma. C3a plays a role in inflammation and immune response and its levels can provide insight into the activation of the classical, alternative, and lectin pathways of the complement system. Elevated C3a levels can be indicative of diseases associated with increased immune activation, such as infections, autoimmune diseases, or inflammatory conditions.
Special Instructions
Ensure that after blood collection, the plasma is separated and frozen as quickly as possible to maintain the integrity of the sample. This test is intended for use in clinical settings and requires proper handling and processing of specimens before submission to ensure accurate results. Additionally, requests for testing should be made through recognized ordering protocols and clinician guidelines.
Limitations
The test is limited to measuring C3a and does not assess other components of the complement system or the overall functional state of immune pathways. Hemolysis can interfere with the test results and cause rejection of the specimen. As such, proper specimen handling and prompt freezing are critical. The test has not been cleared or approved by the FDA and should not be used as the sole basis for diagnosis or clinical decision making. Results should be interpreted in conjunction with clinical findings and other diagnostic tests.
New York Approved
Methodology
Immunoassay (RIA)
Biomarkers
LOINC Codes
- 4488-3
- 4488-3
Result Turnaround Time
21-44 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1.5 mL
Minimum Volume
0.5 mL
Container
Plastic vial
Collection Instructions
Within 30 minutes of collection, centrifuge at 1600 (+/-200) x g (rcf) at 4 degrees C for 15 minutes. Aliquot 1.5 mL of plasma into a plastic vial and freeze immediately at -20 degrees C for up to 4 weeks or at -60 degrees C or below for up to 1 year. Send frozen.
Causes for Rejection
Gross hemolysis
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | 365 days |