C4 Level by RIA
Use
The C4 Level by RIA test is useful for assessing the activation of the complement system, specifically the classical and lectin pathways. The measurement of C4a, an anaphylatoxin, is significant in various clinical conditions where these pathways might be activated, such as autoimmune disorders, infections, and certain types of kidney disease. Elevated levels of C4a can provide valuable information regarding the status and activity of these pathways, aiding in diagnosis and management of related diseases.
Special Instructions
Not provided.
Limitations
The C4 Level by RIA test should not be the sole basis for a clinical diagnosis. Results must be interpreted in conjunction with clinical history, symptoms, and other laboratory findings. Potential pre-analytical variables, such as improper handling leading to hemolysis, can affect results and lead to specimen rejection. The test has not been cleared or approved by the FDA, and is classified as an analyte specific reagent (ASR) test as per the laboratory's validation.
New York Approved
Methodology
Immunoassay (RIA)
Biomarkers
C4a Level
Protein
Result Turnaround Time
9-14 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1.5 mL
Minimum Volume
0.5 mL
Container
Plastic vial
Collection Instructions
Mix well. Within 30 minutes of collection, centrifuge at 1600 (+/-200) x g (rcf) at 4 degrees C for 15 minutes. Aliquot 1.5 mL of plasma into a plastic vial. Freeze plasma immediately at -20 degrees C for up to 4 weeks or, ideally, at -60 degrees C or below for up to 1 year. Send frozen.
Causes for Rejection
Gross hemolysis
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | 365 days |