Calreticulin ex9mut Immunostain, Technical Component Only
Use
The detection of calreticulin exon 9 frameshift alterations can assist in the diagnosis and prognostication of myeloproliferative diseases. Although these alterations are heterogeneous, they all result in a protein with a novel 36-amino acid C terminus the anticalreticulin CAL2 clone specifically identifies. Most patients with essential thrombocythemia or primary myelofibrosis not associated with Janus kinase 2 (_JAK2_) or _MPL_ variants are associated with _CALR_ exon 9 variants and primary myelofibrosis patients carrying _CALR_ variants have a more indolent clinical course.
Special Instructions
This test includes only technical performance of the stain; no pathologist interpretation is provided. Technical component only stains should not be ordered with PATHC / Pathology Consultation. If ordered with PATHC, the technical component stains will be canceled. Any immunohistochemistry (IHC)/in situ hybridization (ISH) stain performed as a part of the PATHC will be performed at the reviewing pathologist's discretion at an additional charge.
Limitations
This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. The positive and negative controls are verified as showing appropriate immunoreactivity. Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick. Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block.
Causes for Rejection
Wet/frozen tissue, Cytology smears, Nonformalin fixed tissue, Nonparaffin embedded tissue, Noncharged slides, ProbeOn slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |