Carbapenemase Detection-Carba NP Test, Varies
Use
This test is useful for confirming carbapenemase production from pure isolates of Enterobacterales or Pseudomonas aeruginosa. Gram-negative bacilli with acquired carbapenemases have disseminated worldwide, creating a global threat. Prompt detection of these enzymes is crucial as carbapenem-resistant infections are associated with elevated mortality rates. Timely identification aids in the management and choice of appropriate therapeutic options.
Special Instructions
Specimen source and organism identification are required. Each isolate must be submitted under a separate order. If not ordering electronically, complete and send a Microbiology Test Request form with the specimen. Place specimen in a large infectious container and label it as an etiologic agent.
Limitations
Results should be interpreted alongside antimicrobial susceptibility testing as phenotypic resistance to carbapenems may occur due to mechanisms other than carbapenemases. The test does not determine the specific type of carbapenemase present. False-negatives may arise from plasmid loss, nonexpressed carbapenemase genes, or low-level expression. It is suggested to prefer molecular detection methods if specific epidemiological markers suggest genes like KPC or NDM carbapenemase presence.
New York Approved
Methodology
Immunoassay (Colorimetric Detection)
Biomarkers
LOINC Codes
- 74676-8
- 74676-8
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
Not provided
Minimum Volume
Not provided
Container
Agar slant or other appropriate media
Collection Instructions
Perform isolation of bacterial isolate. Submit Enterobacterales or Pseudomonas aeruginosa isolate in pure culture actively growing. Do not submit mixed cultures. Place the slant into the secondary infectious container for shipment.
Causes for Rejection
Agar plate
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |