CD14 Immunostain, Technical Component Only
Use
CD14 (cluster of differentiation 14) is a glycosylphosphatidylinositol-linked glycoprotein predominantly found on mature cells of monocytic lineage, such as monocytes, macrophages, and Langerhans cells, as well as follicular dendritic cells. It serves as an important marker for identifying histiocytic/monocytic lineage in acute leukemias. The presence of CD14 can provide valuable information in the assessment of marrow, blood, and tissue samples, aiding in the diagnosis and evaluation of hematological conditions.
Special Instructions
This test includes only the technical performance of the stain and does not provide a pathologist interpretation. It should not be ordered alongside PATHC / Pathology Consultation, as the technical component stains will be canceled if this occurs. Additionally, ensure the use of the specific Immunostain Technical Only Envelope for specimen submission.
Limitations
The test solely comprises the technical aspect of staining procedures without pathologist interpretation. Immunoreactivity can be influenced by several factors such as the age of paraffin sections and the quality of glass slides. Furthermore, environmental factors may affect the charge of the slides and ultimately influence the staining results. For optimal outcomes, paraffin sections should be cut within 6 weeks, and glass slides should be handled with care to avoid exposure to high humidity and heat.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Collect specimen as formalin-fixed, paraffin-embedded tissue block or 2 unstained, positively charged glass slides, sections 4-microns thick.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin-fixed tissue, nonparaffin-embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |