CD31 Immunostain, Technical Component Only
Use
CD31 (cluster of differentiation 31) is a marker of endothelial cells and shows expression on these cells with membrane and occasional cytoplasmic staining. It helps in diagnosing conditions like angiosarcoma, a neoplasm of endothelial cells. It is not expressed in discontinuous endothelium such as the splenic red pulp, but is found on other cell types like megakaryocytes, histiocytes, plasma cells, and certain T-cell subsets.
Special Instructions
Not provided.
Limitations
The test is limited to technical performance of the stain, without pathologist interpretation. The age of a cut paraffin section can significantly affect immunoreactivity, and stability thresholds vary and are antigen dependent. Furthermore, the charge of glass slides is influenced by environmental factors, potentially affecting stain quality.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
CD31
Protein
LOINC Codes
- Order only; no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Preferred specimens include formalin-fixed, paraffin-embedded tissue block or 2 unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered.
Storage Instructions
Attach the green 'Attention Pathology' address label and the pink Immunostain Technical Only label to the outside of the transport container.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient preferred |
| Refrigerated | Refrigerated alternate |