CD35 Immunostain, Technical Component Only
Use
The test is useful for identification of follicular dendritic cells and granulocytes. It aids in the diagnosis of follicular dendritic-cell sarcoma, which involves the involvement of follicular dendritic cells in presenting antigens to B cells within lymphoid follicles.
Special Instructions
This test includes only technical performance of the stain; no pathologist interpretation is provided. Technical component only stains should not be ordered with PATHC / Pathology Consultation, as they will be canceled. If interpretation is required, a separate order for PATHC / Pathology Consultation should be made.
Limitations
Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks. Unsuitable glass slides can result in inconsistent staining due to poor slide surface chemistry. Best practices include minimizing time slides are stored after being unpackaged, limiting exposure to high humidity and heat, and minimizing exposure to plastics.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Formalin-fixed, paraffin-embedded tissue block (preferred) or 2 unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |
| Refrigerated | Refrigerated |