CD68 (PG-M1) Immunostain, Technical Component Only
Use
CD68 (PG-M1) immunostain is used for the identification of monocytic differentiation and phenotyping hematolymphoid neoplasms suspected to represent histiocytic sarcomas. In normal tissues, the stain marks monocytes, macrophages, and neutrophils in a cytoplasmic granular pattern. It is applied to acute leukemia cases to demonstrate monocytic differentiation and to hematolymphoid neoplasms that could be histiocytic sarcomas.
Special Instructions
This test only includes the technical performance of the stain; no pathologist interpretation is provided. The technical component only stains should not be ordered with a Pathology Consultation (PATHC). If ordered with PATHC, the technical component stains will be canceled. Immunostaining is performed at the reviewing pathologist's discretion for PATHC at an additional charge.
Limitations
The age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary among literature and depend on the antigen. Variability in glass slide charge due to environmental factors can alter staining. Best practice is for sections to be cut within 6 weeks and positively charged slides to be handled to minimize exposure to humidity, heat, and plastics.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- Order only;no result
- Bill only; no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Preferred formalin-fixed, paraffin-embedded tissue block or 2 unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Storage Instructions
Attach the green 'Attention Pathology' address label (T498) and the pink Immunostain Technical Only label to the transport container.
Causes for Rejection
Wet/frozen tissue, Cytology smears, Nonformalin fixed tissue, Nonparaffin embedded tissue, Noncharged slides, ProbeOn slides, Snowcoat slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |