CD71 Immunostain, Technical Component Only
Use
The test is useful for the assessment of erythroid lineage by detecting the expression of the transferrin receptor, CD71, which is highly expressed on erythroid cells. It plays a crucial role in mediating the uptake of transferrin-iron complexes essential for erythropoiesis. The expression levels are highest in early erythroid precursors through the intermediate normoblast phase and decrease during the reticulocyte phase.
Special Instructions
This test includes only the technical performance of the immunohistochemical stain and does not include pathologist interpretation. Technical component only stains should not be ordered with PATHC / Pathology Consultation. If ordered with PATHC, the technical component stains will be canceled. Digital images of IHC stains are accessible to clients who place orders electronically.
Limitations
The test does not include pathologist interpretation, and interpretation should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist. The age of cut paraffin sections and the environmental exposure of glass slides can affect the quality of immunoreactivity. Best practice suggests cutting paraffin sections within 6 weeks and minimizing storage time for slides.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Formalun-fixed, paraffin-embedded tissue block or 2 unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4 microns thick.
Storage Instructions
Attach the green 'Attention Pathology' address label and pink Immunostain Technical Only label included in the kit to the transport container.
Causes for Rejection
Wet/frozen tissue, cytology smears, non-formalin fixed tissue, non-paraffin embedded tissue, non-charged slides, ProbeOn slides, Snowcoat slides.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |
| Refrigerated | Refrigerated |