CD8 Immunostain, Technical Component Only
Use
CD8, or cluster of differentiation 8, is a transmembrane glycoprotein primarily expressed in cytotoxic T cells. The identification of CD8 is crucial for diagnosing and classifying lymphomas and chronic lymphoproliferative disorders. The presence of CD8-positive T-lymphocytes can provide important diagnostic and prognostic information, which may influence treatment decisions in various clinical settings including oncology, particularly in the context of tumor-associated lymphocytes, which are often indicative of a favorable prognosis in certain types of cancers.
Special Instructions
Not provided.
Limitations
Age of cut paraffin sections and environmental factors affecting slide chemistry can influence immunoreactivity and staining quality. Stability of antigens may vary widely based on literature, and it is recommended to perform staining within specific thresholds to avoid loss of immunoreactivity. Slides should be stored to minimize exposure to heat and humidity, and unsuitable slides may lead to inconsistent staining results. Best practice is to use new positively charged slides and avoid materials that are prone to alterations due to storage conditions.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
CD8
Protein
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Submit formalin-fixed, paraffin-embedded tissue block or two unstained, positively charged glass slides (4-micron thickness) per test ordered in the Immunostain Technical Only Envelope.
Storage Instructions
Store slides in a dry environment to avoid humidity exposure which may affect slide quality.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |