Chikungunya Virus, PCR, Molecular Detection, Serum
Use
The test is used for the qualitative detection of chikungunya virus RNA from serum collected during the acute phase of infection. It is intended for evaluating patients with a clinical history and symptoms consistent with chikungunya virus infection. This test is not recommended for screening healthy patients. The chikungunya virus is an RNA virus transmitted primarily by infected mosquitoes, and infection may be suspected based on symptoms such as fever, joint pain, and headache, particularly after recent travel to areas where the virus is endemic.
Special Instructions
Due to the short period in which chikungunya RNA may be detected in serum, it is recommended to also test serum for IgM- and IgG-class antibodies to chikungunya virus. Additionally, testing for RNA or IgM-class antibodies to dengue and Zika viruses should be considered.
Limitations
Negative results do not preclude chikungunya virus infection and should not be used as the sole basis for treatment or management decisions. False-negative results may occur due to RNA degradation during improper shipping or storage. Specimen collection after the typical detection period (7 days post-symptom onset) may also lead to false negatives.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 81152-1
- 81152-1
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.3 mL
Container
Sterile container
Collection Instructions
Within 2 hours of collection, centrifuge and aliquot the serum into a sterile container. Serum specimens not aliquoted from the serum gel collection tube into a sterile container will be rejected.
Causes for Rejection
Gross hemolysis, Heat-inactivated specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |