Chlamydia IgM, Immunofluorescence, Serum
Use
The test is used for assessing IgM antibody levels to help in diagnosing infections caused by Chlamydia pneumoniae or Chlamydia psittaci. These organisms are part of the Chlamydiaceae family and are obligate intracellular pathogens. Chlamydia pneumoniae causes pneumonia in humans and is spread human to human, while Chlamydia psittaci is transmitted via birds and leads to psittacosis, characterized by pneumonia and other systemic symptoms.
Special Instructions
The test is performed using a microimmunofluorescent antibody assay. It is important to collect the specimen in the correct type of container and to ensure the sample is not hemolyzed, lipemic, or icteric which can lead to rejection. It's also crucial to adhere to proper specimen stability guidelines for accurate results.
Limitations
The test may show limited sensitivity and specificity, particularly in distinguishing between Chlamydia species. Cross-reactivity with other Chlamydia species can occur early in the infection. If infection is suspected but antibodies are not detected, it may be necessary to collect an additional specimen after 10 to 21 days. The test does not detect antibodies against Chlamydia trachomatis; molecular methods are recommended for such cases.
New York Approved
Methodology
Immunoassay (Micro-Immunofluorescent Antibody (MIF) Assay)
Biomarkers
LOINC Codes
- 10849-8
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.3 mL
Minimum Volume
0.15 mL
Container
Serum gel preferred or Red top acceptable, Submission in a plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Causes for Rejection
Gross hemolysis, lipemia, icterus; heat-inactivated specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 30 days |
| Frozen | 30 days |