Coxiella burnetii (Q Fever), Molecular Detection, PCR, Varies
Use
The test is useful for aiding in the diagnosis of Coxiella burnetii infection, also known as Q fever, by detecting the presence of Coxiella burnetii DNA using molecular methodologies. Q fever is caused by the intracellular bacterium and is typically associated with a mild respiratory disease, but can lead to chronic infections such as endocarditis if untreated. The real-time PCR target is the shikimate dehydrogenase gene, unique to C. burnetii, making it a specific method for the detection of this pathogen in clinical specimens.
Special Instructions
Specimen source is required for testing. The test must be ordered with the appropriate Microbiology Test Request form if not ordered electronically.
Limitations
A negative PCR result does not rule out the presence of Coxiella burnetii DNA if it is below the limit of detection, if there is inhibition of PCR, or if there are sequence variabilities under the primers or probes. Test results should be considered as part of a diagnostic tool and not used as the sole basis for diagnosis. Correlation with patient symptoms and clinical presentation is essential.
New York Approved
Methodology
PCR-based (Real-Time PCR)
Biomarkers
LOINC Codes
- 90442-5
- 31208-2
- 90442-5
Result Turnaround Time
2-7 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Entire collection or 5 mm(3)
Minimum Volume
5 mm(3)
Container
Sterile container
Collection Instructions
Collect fresh tissue specimen. Submit tissue only, do not add fluid to tissue. Refrigerate or freeze specimen.
Storage Instructions
Refrigerate or freeze specimen.
Causes for Rejection
Tissue in formalin formaldehyde, acetone; Bone marrow; Decalcified bone; Unstained slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |
| Refrigerated | <7 days |
| Frozen | <7 days |