Cyclosporine, Blood
Use
Cyclosporine testing is crucial for monitoring whole blood cyclosporine concentrations in patients undergoing therapy, especially for those coadministered with CYP3A4 substrates, inhibitors, or inducers. It helps in adjusting doses to optimize immunosuppression and minimize toxicity, as well as assessing patient compliance. Cyclosporine is used post solid organ transplantation to suppress T-cell activation, decreasing IL-2 production. Regular monitoring is vital due to its narrow therapeutic range and variable pharmacokinetic parameters affecting absorption and half-life.
Special Instructions
Collect specimen immediately before a scheduled dose without centrifuging or aliquoting. Send whole blood specimen in the original tube. Use Therapeutics Test Request or Kidney Transplant Test Request forms if not ordered electronically.
Limitations
The test has limitations in diagnosing using samples drawn other than at trough levels; results will be higher if not collected immediately before the next dose. The therapeutic range applies only to trough samples, and variations in the therapeutic range depend on the type of transplant and accompanying drugs. The test is specific for cyclosporine but may not reflect the presence of metabolites or concurrent drugs like sirolimus or tacrolimus, whose metabolites could interact with cyclosporine.
New York Approved
Methodology
Mass Spectrometry (LC-MS/MS)
Biomarkers
LOINC Codes
- 3520-4
- 3520-4
Result Turnaround Time
0-1 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
3 mL
Minimum Volume
1 mL
Container
Lavender top (EDTA)
Collection Instructions
Collect specimen immediately before a scheduled dose. Do not centrifuge. Send whole blood specimen in original tube. Do not aliquot.
Causes for Rejection
Clotted specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |