Cytomegalovirus (CMV) CD8 T-Cell Immune Competence, Quantitative Assessment by Flow Cytometry, Blood
Use
This test is useful for assessing cytomegalovirus (CMV)-specific immune competence in allo-hematopoietic stem cell transplantation patients who are at risk for developing late CMV disease (beyond day 100 after transplant) and in solid organ transplant patients who are at high risk for CMV reactivation posttransplant. It is also useful for monitoring immune competence in patients post-primary CMV infection after transplant who are at risk for CMV reactivation after the cessation of antiviral prophylaxis. Additionally, it helps identify individuals who are likely to be protected from posttransplant CMV infection and those who are at higher risk of CMV reactivation. The global CD8 T cell immune competence assay determines over immunosuppression within the CD8 T cell compartment, useful for transplant recipients and patients with autoimmune disorders receiving immunosuppressant therapy.
Special Instructions
Ordering Guidance: Patient must be cytomegalovirus seropositive and have 1 or more of the 5 major histocompatibility complex alleles: HLA A1, A2, B7, B8, or B35 to utilize this assay. Additional Testing Requirements: It's important to ascertain the patient's and donor's cytomegalovirus serostatus, as well as the patient's major histocompatibility complex class I HLA haplotype, before ordering this assay.
Limitations
The assay is specific for 5 major histocompatibility complex (MHC) class I alleles: HLA A1, A2, B7, B8, and B35, which cover about 70% of the White population and approximately 40% of the Asian population. Serial measurements are essential to accurately assess immune response. CD8 T cell immune competence assessment is specific to CD8 T cells and does not provide information about overall T cell competence. Further studies are needed to determine if certain levels of IFN-gamma and CD107a/b expression confer varying degrees of risk for infectious disease.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 95184-8
- 95191-3
- 95190-5
- 95189-7
- 95188-9
- 95187-1
- 95203-6
- 95193-9
- 95197-0
- 95196-2
- 95195-4
- 95194-7
- 95204-4
- 95186-3
- 95192-1
- 69052-9
- 95185-5
- 95202-8
- 95201-0
- 95200-2
- 95199-6
- 95198-8
- 8122-4
- 14135-8
Result Turnaround Time
3-6 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
20 mL
Minimum Volume
10 mL
Container
Green top (sodium heparin)
Collection Instructions
Send whole blood specimen in original tube. Do not aliquot. Collect and package specimen as close to shipping time as possible. Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668). It is recommended that specimens arrive within 24 hours of collection.
Causes for Rejection
Gross hemolysis, Gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |